Effects of Caffeine Ingestions Concomitant to Incremental Running Test on Lipid Peroxidation and Enzymatic Antioxidant in Active Men

Document Type : Original Article

Abstract

Purpose: The aim of the current research was to assess effects of caffeine (5 and 9 mg.kg -1 ) ingestions 
concomitant to incremental running test on malondialdehyde (MDA) as lipid peroxidation marker and 
superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) as enzymatic antioxidants. 
Material and Methods: Ten subjects were tested on a treadmill until exhaustion on three separate occasions 
between which there were intervals of 5 days. Every volunteer underwent three conditions containing placebo 
or caffeine doses that were consumed one hour before exercise (pre-test). Blood samples were collected pretest, before caffeine or placebo consumption and immediately after exercise (post-test). 
Results: Results showed that in comparison to placebo condition, caffeine doses decreased MDA levels and 
increased GPx activities significantly (P< 0/05). Regardless of 5 mg.kg -1 caffeine ingestion that had no 
significant effect on CAT and SOD activities (P< 0/05), 9 mg.kg -1 caffeine intake significantly increased and 
decreased CAT and SOD respectively (P< 0/05), when compared to placebo. For MDA levels, there was no 
significant difference between caffeine doses (P< 0/05), but these differences for CAT, SOD and GPx were 
significant (P< 0/05). 
Discussion and Conclusion: In conclusion caffeine doses approximately had equal effects on lipid 
peroxidation decline, while these effects on enzymatic antioxidant with 9 mg.kg -1 caffeine intake were better 
than 5 mg.kg-1 caffeine

Keywords

• Oxidative stress
• Free radicals
• Progressive exercise
• Bruce protocol
• Caffeine doses

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