Effect of 8 Weeks Treadmill Running with or without Pistachia Atlantica Liquid Extraction on Liver ABCG8 Gene Expression and Cholesterol Level in Female Rat

Document Type : Original Article

Abstract

Introduction: Reverse cholesterol transport (RCT) is a term used to describe the efflux of excess cellular 
cholesterol. ABCG8 is a member of the ABCG family that play a critical role in this process. It has been 
reported that several factors (nutrients, fasting and physical stress) could affect on gene expression. Thus, the 
current study was conducted to investigate the effect of endurance exercise with or without Baneh extraction 
on the expression of liver ABCG8 in female rat.
Material and Methods: In this study twenty wistar rats (six to eight weeks old, 125-135 g weight) were 
used. Animals were randomly assigned into training (n = 10) and control (n = 10) groups and further divided 
into saline-control (SC), saline-training (ST), and Baneh-control (BC), and Baneh-training (BT). Training 
groups was given exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day, 5 days/week 
for eight weeks. Animals were fed as the oral with Baneh extraction and saline for four week. 72 hours after 
the last training session and after four hours fasting, rats were sacrificed and liver total RNA isolated, after 
PCR procedure, ABCG8 gene expression was detected by Real-time PCR method. Statistical analysis were 
performed using a one way analysis of variance and significance was accepted at P < 0.05. 
Results: Results demonstrated that independently Baneh extraction reduce ABCG8 gene expression in liver 
(P<0.013). Exercise training increase ABCG8 relative gene expression than BC (P<0.016) and BT (P<0.011) 
groups; however exercise don’t have independent significant effect on ABCG8 fold change at the end of the 
program (P<0.27). 
Discussion and Conclusion: Our findings show that hepatic cholesterol in saline-control group significantly 
was lower than Baneh groups (P<0.001) and there wasn’t significant change between other groups.

Keywords

• Endurance exercise
• Reverse cholesterol transport
• Cellular cholesterol efflux

---